Useful Links & Tools
- MSDS
- Specification Sheet
- Certificate of Analysis
- Certificate of Origin
- T6674 - Datasheet (86KB)
- Celltransmissions 19(3) (162 KB)
- Celltransmissions 20(1) (708 KB )
- Celltransmissions 19(1) (143 KB )
T6674
Sigma
Tumor Necrosis Factor-α human
recombinant, expressed in Escherichia coli, powder, cell culture tested
| Synonym: | TNF-α |
| CAS Number: | 94948-59-1 |
| MDL number: | MFCD00148692 |
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Analysis Note | The biological activity of TNF-α is measured by the cytolysis of murine L929 cells. |
| Physical form | Lyophilized from a 0.2 μm-filtered buffer solution containing 3mM Tris-HCl and 5% trehalose. |
| Biochem/physiol Actions | Tumor necrosis factor-α, also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated. |
Properties
| recombinant | expressed in Escherichia coli |
| assay | ≥97% (SDS-PAGE) |
| form | powder |
| potency | 0.02-0.3 ng/mL ED50/EC50 |
| mol wt | mol wt ~17.4 kDa |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| Gene Information | human ... TNF(7124) |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Ware, C., et al., Thomson, A.W., ed., Tumor necrosis factor-related ligands and receptors. The Cytokine Handbook 3rd ed., San Diego, CA , (1998), 549 |
| Aggarwal, B., and Reddy, S., Nicola, N., ed., Tumor necrosis factor (TNF). Guidebook to Cytokines and Their Receptors New York , (1994), 103-104 | |
| Callard, R., and Gearing, A. The Cytokine Facts Book New York , (1994) | |
| Beutler, B., Sporn, M., and Roberts, A, ed., Cachectin/tumor necrosis factor and lymphotoxin. Peptide Growth Factors and their Receptors II New York , (1991), 39-70 | |
| Clemens, M. J., et al. Lymphokines and Interferons, A Practical Approach, (1994), 121 |
